Biotin Anti-CD8a Monoclonal Antibody for Flow Cytometry - P01732

Core Product Specifications and Parameters

Biological Background: CD8a (T-cell surface glycoprotein CD8 alpha chain) Function and Localization

• CD8a is an integral membrane glycoprotein that acts as a coreceptor for MHC class I molecules on T-cells, enabling recognition of peptide antigens derived from cytosolic proteins.
• It interacts simultaneously with the T-cell receptor (TCR) and MHC class I on antigen-presenting cells, recruiting the Src kinase LCK to initiate signaling cascades that lead to T-cell activation, lymphokine production, and cytotoxicity.
• CD8a forms either heterodimers with CD8B on thymus-derived T-cells or homodimers (CD8a/CD8a) on a subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• The protein is localized to the cell membrane, but a secreted form also exists, likely derived from alternative splicing.
• Key post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation, which are important for its structure and membrane association.
• CD8a has an immunoglobulin-like domain and is part of the adaptive immune system, with roles in thymic selection and the generation of memory CD8 T-cells.
• Disease variants in CD8A are associated with immunodeficiencies, highlighting its critical role in immune defense.

Experimental Guidance and Technical Tips

• The antibody is generated against recombinant protein corresponding to the extracellular domain (aa 22-182) of human CD8A, which includes the critical MHC class I binding region; consider confirming specificity using CD8a-expressing and CD8a-knockout cell lines in flow cytometry.
• For flow cytometry applications, optimal staining concentrations should be titrated using relevant primary cells (e.g., human PBMCs or cynomolgus monkey lymphocytes) due to variability in antigen density; a biotin-streptavidin detection system may amplify sensitivity.
• Cross-reactivity with cynomolgus monkey CD8a allows for translational studies, but verify performance in your specific assay system as epitope conservation may vary.
• Since CD8a is expressed on multiple immune subsets, gating strategies should incorporate additional markers (e.g., CD3, CD4) to distinguish cytotoxic T-cells from NK cells and memory populations.
• For intracellular staining, permeabilization may affect epitope accessibility; consider testing fixation/permeabilization protocols with positive and negative controls.

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CamelBio provides comprehensive IVD raw material solutions, connecting diagnostic manufacturers and research laboratories with validated antibody reagents like this anti-CD8a monoclonal antibody for immuno-oncology and T-cell profiling. Beyond single products, we offer one-stop access to matched antibody pairs, bulk ancillary reagents, and tailored technical support to accelerate assay development from concept to clinic.