Biotin Anti-CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (PTPRC, L-CA, T200) is a receptor-type tyrosine-protein phosphatase essential for T-cell activation through the antigen receptor.
  Related references:
  PMID:35767951

• The protein contains two intracellular phosphatase domains; the membrane-proximal domain possesses catalytic activity, while the distal domain modulates substrate specificity.

• CD45 dephosphorylates key signaling mediators such as SKAP1, FYN, and LYN, and interacts with CLEC10A on dendritic cells at the immunological synapse to fine-tune T-cell activation thresholds and prevent autoreactivity.

• It serves as a receptor for human cytomegalovirus protein UL11, leading to impaired TCR signaling and reduced T-cell proliferation upon infection.

• Multiple isoforms (131–147 kDa) are generated by alternative splicing; thymocyte expression is restricted to isoforms 1, 2, 3, 5, and 7.

• The protein localizes predominantly to the cell membrane, with enrichment in lipid rafts and at the synapse, reflecting its role in signal transduction.

• Keywords from UniProt include alternative splicing, glycoprotein, host-virus interaction, phosphoprotein, and membrane, highlighting its structural and functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 26–577) of human CD45, which includes the alternatively spliced domains; be aware that the antibody may recognize multiple isoforms depending on the epitope.
• As a biotin-conjugated antibody, it is designed for direct detection with streptavidin–fluorophore conjugates in flow cytometry—optimize antibody and streptavidin titrations for your specific cell system.
• The antibody cross-reacts with cynomolgus monkey CD45, making it suitable for translational studies using non-human primate models; validate performance in the relevant species and tissue.
• For intracellular phospho-flow applications, consider whether the biotin–streptavidin system introduces background and test appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research groups streamlined access to IVD raw materials, including validated monoclonal antibodies like this anti-CD45 reagent for immunology and infectious disease research. Beyond single products, we provide matched antibody pairs, bulk ancillary reagents, and sourcing for rare-target reagents—all backed by technical consultation from concept to clinic to accelerate your diagnostic development.