Biotin Anti-CD4 Monoclonal Antibody for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a T-cell surface glycoprotein (also known as T4/Leu-3) encoded by the CD4 gene, belonging to the immunoglobulin superfamily, and plays a central role in adaptive immunity.
• As a coreceptor, CD4 binds MHC class II molecules on antigen-presenting cells and interacts with the T-cell receptor (TCR) to recruit the Src kinase LCK, initiating intracellular signaling cascades that promote T-helper cell activation, lymphokine production, adhesion, and motility.
• CD4 participates in thymic T-helper cell development and triggers the differentiation of monocytes into mature functional macrophages, highlighting its broader role in immune cell maturation.
• It is an integral membrane glycoprotein localized at the cell membrane, with extensive post-translational modifications including disulfide bonds, glycosylation, palmitoylation, and phosphorylation.
• CD4 is highly expressed in T-helper cells and serves as a defining marker for this subset; it is also present on other immune cells such as macrophages, dendritic cells, and NK cells.
• CD4 is the primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:12089508, PubMed:16331979, PubMed:2214026) and human herpesvirus-7 (HHV-7) (PubMed:7909607). HIV-1 Vpu protein mediates CD4 down-regulation (PubMed:17346169). Related references: PMID:12089508 PMID:16331979 PMID:2214026 PMID:17346169 PMID:7909607

Experimental Guidance and Technical Tips

• The biotin label enables direct detection without secondary antibodies; consider using fluorophore-conjugated streptavidin for flow cytometry (FC) to amplify signal.
• This antibody is raised against recombinant human CD4 protein; cross-reactivity with cynomolgus monkey has been observed, but validation in the relevant sample system is recommended due to potential sequence differences.
• For FC, perform antibody titration to determine the optimal concentration that yields the best signal-to-noise ratio, and include appropriate isotype controls.
• As a rabbit-derived monoclonal antibody, be mindful of potential Fc receptor interactions when staining human myeloid cells; blocking steps may be considered.
• CD4 is a cell surface protein; for intracellular staining, optimize fixation and permeabilization conditions to preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and consulting expertise—from concept to clinic. For CD4-focused immune phenotyping and HIV research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated supply model is designed to accelerate your assay development and streamline procurement, ensuring you have the critical tools needed for immune function and infectious disease studies.