Biotin Anti-CD4 Monoclonal Antibody for FC - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• Encoded by the CD4 gene, T-cell surface glycoprotein CD4 is a single-pass type I membrane protein and a member of the immunoglobulin superfamily.
• Serves as a critical coreceptor during T-cell activation by binding to MHC class II molecules on antigen-presenting cells, thereby stabilizing the TCR–peptide–MHC complex and recruiting the tyrosine kinase LCK to initiate intracellular signaling cascades.
• Highly expressed on T-helper cells, with additional presence on regulatory T cells, monocytes, macrophages, dendritic cells, and NK cells, defining a key immunophenotypic marker for CD4+ lymphocyte subsets.
• Localized at the cell membrane, where its extracellular domain mediates ligand interactions and its cytoplasmic tail couples to signaling machinery.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, palmitoylation, and phosphorylation, which regulate protein stability and signaling competence.
• Acts as the primary receptor for human immunodeficiency virus-1 (HIV-1) and as a receptor for Human Herpesvirus 7 (HHV-7), facilitating viral entry and pathogenesis. Related references: PMID:12089508 PMID:16331979 PMID:2214026 PMID:17346169 PMID:7909607

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the extracellular domain of human CD4, preserving native epitopes recognized by this monoclonal antibody for reliable surface staining.
• This biotin-conjugated antibody is suitable for direct or indirect flow cytometry detection; consider pairing with fluorophore-labeled streptavidin for flexible multicolor panel design.
• For optimal resolution, titrate the antibody on relevant human or cynomolgus lymphocyte populations and include appropriate isotype controls to assess non-specific binding.
• CD4 surface expression can be influenced by cell activation state or HIV-1 infection; validate staining patterns in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers with streamlined access to high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-CD4 monoclonal antibody supports immunophenotyping and infectious disease research, complementing our broad portfolio of CD markers and immune-related targets. When rare or custom targets arise, our one-stop sourcing model helps bridge gaps from discovery to clinical application—contact us to discuss your specific needs.