Bid Rabbit pAb - P55957

Core Product Specifications and Parameters

Biological Background: Bid Function and Localization

• BID (BH3-interacting domain death agonist) is a pro-apoptotic member of the Bcl-2 family that promotes caspase activation and apoptosis. Related references: PMID:14583606
• Upon cleavage by caspases, BID translocates to the mitochondrial outer membrane, disrupting it and releasing cytochrome c to induce cell death. Related references: PMID:15661737, PMID:32029622, PMID:10480954, PMID:9727491
• It counters the protective effect of BCL2, promoting apoptosis.
• BID is primarily cytoplasmic under basal conditions but targets the mitochondrial membrane upon apoptotic stimulation.
• The protein is expressed in spleen, pancreas, placenta, and lung tissues.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitination, regulate BID activity and stability.
• Alternative splicing produces multiple isoforms, adding functional diversity.
• Also known as p22 BID, its molecular weight is approximately 22 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human BID (aa 1-195); therefore, the antibody is likely to recognize both full-length and cleaved forms. Consider running positive controls with apoptotic inducers to observe cleavage products.
• For Western blot, the predicted band is around 22 kDa; validate in the relevant sample system and consider loading controls.
• IF/ICC may require cell permeabilization and apoptotic stimulation to detect mitochondrial translocation. Optimize fixation and staining conditions for each cell type.
• ELISA applications can be developed using the antibody as capture or detection reagent; cross-reactivity with mouse and rat BID allows for multi-species assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated partner for IVD raw material sourcing, providing high-quality antibodies, validated antibody pairs, and ancillary reagents. For apoptosis research and diagnostic applications targeting BID, we offer reliable access to this anti-Bid rabbit polyclonal antibody and can assist with sourcing additional rare-target reagents. Contact us to streamline your procurement and accelerate your assay development from concept to clinic.