BHMT Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q93088

Core Product Specifications and Parameters

Biological Background: BHMT Function and Localization

• BHMT is a betaine--homocysteine S-methyltransferase that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, playing a central role in homocysteine metabolism.
• The enzyme is essential for the irreversible oxidation of choline, linking one-carbon metabolism and methyl group donation.
• BHMT is predominantly expressed in the liver and kidney.
• Subcellular localization includes the cytoplasm, cytosol, and nucleus.
• The protein contains zinc-binding sites and is classified as a metal-binding methyltransferase, requiring zinc for activity.
• Post-translational modifications include phosphorylation; it is identified as a phosphoprotein.
• Structural studies (3D-structure) provide insights into its enzymatic mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 306-406) of human BHMT, ensuring target specificity. Confirm reactivity in your model system by using lysates from BHMT-expressing tissues (liver/kidney).
• For Western blotting under denaturing conditions, a band near 45 kDa is predicted. Use appropriate molecular weight markers and load sufficient protein from target tissues.
• In IHC-P, include a positive control slide of human liver tissue and optimize antigen retrieval conditions. Validate staining specificity with an isotype control or blocking peptide if available.
• Since cross-reactivity with rat is reported, this antibody may be suitable for rodent studies; nevertheless, verification by sequence alignment and experimental validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material provider, offering a comprehensive portfolio of validated antibodies, bulk reagents, and customized sourcing services. For researchers investigating homocysteine metabolism and liver/kidney biomarkers, we supply high-quality monoclonal antibodies like this BHMT antibody to support diagnostic development from concept to clinic. From monoclonal/polyclonal antibodies to enzyme conjugates and ancillary reagents, CamelBio provides one-stop access to essential raw materials, backed by technical support for assay optimization and rare target sourcing.