BGN Rabbit Polyclonal Antibody for WB, ELISA - P21810

Core Product Specifications and Parameters

Biological Background: Biglycan Function and Localization

• Biglycan (BGN), also known as bone/cartilage proteoglycan I or PG-S1, is a member of the small leucine-rich repeat proteoglycan (SLRP) family.
• It is predominantly expressed in connective tissues, with high abundance in articular cartilage, and has been detected in placenta (PubMed:32337544). Related references: PMID:32337544
• Biglycan is believed to play a role in the assembly of collagen fibers, contributing to the structural organization of the extracellular matrix.
• The protein is secreted into the extracellular space and localizes within the extracellular matrix.
• BGN undergoes post-translational modifications including glycosylation and disulfide bond formation, and contains a signal peptide for secretion.
• Genetic variants in BGN are associated with human pathologies such as aortic aneurysm and skeletal dysplasia (dwarfism).

Experimental Guidance and Technical Tips

• When using this rabbit polyclonal antibody for Western blot, a band around 42 kDa is expected for human Biglycan. Consider using appropriate positive controls such as human cartilage or placenta lysates.
• For ELISA, optimize coating antigen and detection conditions; the antibody may be suitable for both direct and indirect formats.
• Validate cross-reactivity with other species if needed, as only human reactivity is currently specified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers with reliable access to IVD raw materials, including this BGN rabbit polyclonal antibody for extracellular matrix and skeletal disease research. Alongside validated antibodies, we offer customized monoclonal development, antigen production, and bulk reagent supply to accelerate your assay development from concept to clinic.