BCL6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 is a zinc finger and BTB domain-containing transcriptional repressor (also known as BCL-5, LAZ-3, ZBTB27, ZNF51) that plays a central role in germinal center formation and antibody affinity maturation.
• It represses target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' or indirectly by interacting with transcription factors, and recruits corepressors and histone deacetylases to mediate repression.
• In germinal center B-cells, BCL6 suppresses genes involved in differentiation, apoptosis, inflammation, and cell cycle control, enabling rapid proliferation and tolerance of DNA breaks during class switch recombination and somatic hypermutation without inducing p53-dependent apoptosis.
• BCL6 also promotes the development of follicular helper T-cells while inhibiting TH1, TH2, and TH17 differentiation, and is required for immunological memory in both T- and B-cells.
• It localizes exclusively to the nucleus and is expressed predominantly in germinal center T- and B-cells and primary immature dendritic cells.
• Additional functions include suppression of macrophage proliferation by competing with STAT5 for binding motifs, control of cell cycle arrest upon genotoxic stress, and regulation of neurogenesis via NOTCH target gene silencing.
• Keywords: Proto-oncogene, involved in chromosomal rearrangements in lymphomas, contains zinc-finger motifs, acetylation, phosphorylation, ubiquitination, and functions as both repressor and activator.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-280 of human BCL6 (NP_001124317.1). This N-terminal region includes the BTB/POZ domain critical for corepressor recruitment; target recognition is expected to be specific to the full-length protein.
• For Western blotting, a band around 79kDa (predicted) may be observed; post-translational modifications such as acetylation or phosphorylation could result in slight mobility shifts. Consider using nuclear extracts from germinal center B-cell lines (e.g., Ramos, Daudi) as positive controls.
• In immunofluorescence/ICC, expect nuclear localization. Co-staining with B-cell markers (e.g., CD20) or germinal center markers (e.g., BCL6 itself, CD10) in lymphoid tissues may help confirm specificity.
• For ELISA applications, the antibody can be used with either the synthetic peptide or recombinant BCL6 protein as capture antigen. Titration and optimization of blocking conditions are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supply platform offering a comprehensive portfolio for diagnostic development—from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents. Our BCL6 antibody supports immunology and oncology research, particularly in B-cell lymphomagenesis and germinal center biology. We also accommodate customized sourcing requests for rare targets, ensuring a seamless bridge from concept to clinic.