BCAS2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75934

Core Product Specifications and Parameters

Biological Background: BCAS2 Function and Localization

• BCAS2, also known as SPF27 or DAM1, is a component of the activated spliceosome required for pre-mRNA splicing (PubMed:28076346, PubMed:28502770, PubMed:29301961). Related references: PMID:28076346, PMID:28502770, PMID:29301961
• It is an integral part of the PRP19-CDC5L complex, which is essential for spliceosome activation and may have a scaffolding role by contacting all core complex components.
• The PRP19-CDC5L complex has been implicated in the response to DNA damage (DDR).
• BCAS2 localizes predominantly to the nucleus, including the nucleolus.
• It is ubiquitously expressed across tissues.
• Post-translational modifications include acetylation and phosphorylation.
• Key biological keywords: mRNA processing, mRNA splicing, spliceosome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–225); this design may support detection of full-length BCAS2. Verify reactivity with your specific sample types.
• For Western blotting, anticipate a band near 26 kDa. Optimize loading and transfer conditions for best signal.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), expect nuclear staining patterns. Antigen retrieval and blocking steps may require optimization.
• When using ELISA applications, standard curves with recombinant protein or lysate controls are recommended to calibrate sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with validated antibody pairs, custom monoclonals/polyclonals, and bulk ancillary reagents for streamlined IVD development. This BCAS2 antibody supports splicing and cancer research programs, and we offer complementary raw materials and technical guidance for assay optimization. Reach out for tailored sourcing solutions to accelerate your project from concept to clinic.