BAZ1A Rabbit Polyclonal Antibody for WB, ELISA - Q9NRL2

Core Product Specifications and Parameters

Biological Background: BAZ1A Function and Localization

• BAZ1A, also known as hACF1, WCRF180, or hWALp1, serves as a regulatory subunit of the ATP-dependent ACF-1 and ACF-5 ISWI chromatin remodeling complexes. These complexes organize nucleosome arrays and slide histone octamers to regulate DNA accessibility during replication, transcription, and repair. Related references: PMID:17099699 PMID:28801535
• The ACF-1 complex has a lower ATP hydrolysis rate than ACF-5, and BAZ1A functions as a linker DNA length sensor to modulate nucleosome spacing activity. Related references: PMID:17099699 PMID:28801535
• BAZ1A, together with SMARCA5/SNF2H, is required for replication of pericentric heterochromatin in S-phase. Related references: PMID:12434153
• It may play a role in nuclear receptor-mediated transcription repression. Related references: PMID:17519354
• BAZ1A localizes predominantly to the nucleus.
• It is highly expressed in testis, with low or undetectable levels in other tissues.
• The protein features a bromodomain, a zinc finger, and coiled coil motifs, and undergoes post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1347-1556 near the C-terminus of human BAZ1A. When designing experiments, consider that this region may be conserved across species, consistent with reported cross-reactivity with mouse and rat.
• For Western blotting, BAZ1A is a large protein (~179 kDa). Use low-percentage (e.g., 6-8%) SDS-PAGE gels and prolonged wet transfer (or semi-dry with appropriate conditions) to ensure efficient transfer.
• In ELISA, the recombinant protein immunogen can serve as a positive control for direct or indirect ELISA assays. Validate the antibody's performance in your specific sample matrix and application.
• Given BAZ1A's role in chromatin remodeling, nuclear extracts or chromatin fractions may be suitable sample types for detection. Confirm specificity by performing knockdown or knockout controls where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for chromatin biology and transcription research. Whether you need ready-to-use BAZ1A antibodies or custom development for rare targets, our supply chain bridges your concept to clinic.