BATF3 Rabbit Polyclonal Antibody for WB and ELISA - Q9NR55

Core Product Specifications and Parameters

Biological Background: BATF3 Function and Localization

• BATF3 (Basic leucine zipper transcriptional factor ATF-like 3), also known as SNFT, JDP1, and JUNDM1, is a member of the AP-1 family of transcription factors.
• It plays a critical role in the immune system by controlling the differentiation of CD8(+) thymic conventional dendritic cells and is essential for the development of CD8-alpha(+) classical dendritic cells (cDCs) and CD103(+) dendritic cells.
• These dendritic cell subsets are specialized for cross-presenting antigens to CD8 T-cells and producing interleukin-12 (IL12) in response to pathogens.
• BATF3 forms a heterodimer with JUN family proteins (e.g., c-Jun) and binds to the DNA sequence 5'-TGA[CG]TCA-3' (TPA response element, TRE) to regulate target gene expression.
• The protein localizes to the nucleus, consistent with its function as a transcriptional regulator.
• Post-translational modifications include phosphorylation, as indicated by the keyword 'Phosphoprotein'.
• Additional keywords from UniProt include 'Activator', 'Repressor', 'DNA-binding', and 'Transcription regulation', reflecting its dual role in gene activation and repression.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant protein corresponding to amino acids 1-127 of human BATF3, which covers the full-length protein. Consider this when planning epitope-specific detection.
• For Western blot (WB), use whole-cell lysates from human dendritic cells or relevant cell lines; validate the antibody in the sample system of interest to confirm specificity.
• Cross-reactivity with rat has been observed, so this antibody may be suitable for rat samples; however, verify performance in rat-derived tissues or cells.
• For ELISA, consider using recombinant BATF3 protein as a standard and optimize coating conditions; note that direct ELISA performance may require additional validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including this BATF3 polyclonal antibody for immune cell research. Our portfolio covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to streamline your development pipeline from concept to clinic with reliable procurement and supply solutions.