BANK1 Rabbit Polyclonal Antibody for WB, ELISA - Q8NDB2

Core Product Specifications and Parameters

Biological Background: BANK1 Function and Localization

• BANK1 is a B-cell scaffold protein containing ankyrin (ANK) repeats, playing a critical role in B-cell receptor (BCR) signal transduction.
• It functions primarily in BCR-induced calcium (Ca²⁺) mobilization from intracellular stores, a key event for B-cell activation.
• BANK1 promotes Lyn-mediated tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors (IP3R1 and IP3R2), facilitating calcium release.
• The protein is predominantly expressed in B lymphocytes, with highest levels in CD19⁺ B-cells, and is not detected in T-cell or myeloid lineages.
• Alternative splicing of BANK1 generates multiple isoforms, expanding functional diversity.
• As a phosphoprotein, BANK1 undergoes post-translational modifications that modulate its activity in immune signaling.
• Genetic variations in BANK1 are linked to systemic lupus erythematosus (SLE), highlighting its role in autoimmunity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 516-785), which may be conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, expect a band near 89 kDa; actual migration can vary due to post-translational modifications or isoform expression.
• Validate antibody specificity in B-cell lines or primary CD19⁺ B-cells, given the restricted expression pattern.
• Consider performing calcium flux assays in B-cells to correlate detection with functional BCR signaling events.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials. This BANK1 antibody serves B-cell immunology and autoimmunity research, complementing our offerings of validated antibody pairs, recombinant proteins, and ancillary reagents. For custom or bulk needs in rare target sourcing, CamelBio provides streamlined procurement from concept to clinic.