BAG3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O95817

Core Product Specifications and Parameters

Biological Background: BAG3 Function and Localization

• BAG3 acts as a co-chaperone that bridges HSP70 family chaperones (e.g., HSPA1A, HSPA8) with small heat shock proteins such as HSPB8, facilitating the recruitment of the autophagy machinery. Related references: PMID:27884606, PMID:30559338
• It functions as a nucleotide exchange factor (NEF) for HSP70s, accelerating ADP release from the nucleotide-binding domain (NBD) and promoting client release through interactions with the substrate-binding domain (SBD). Related references: PMID:27474739, PMID:9873016
• BAG3 exhibits anti-apoptotic properties, interacting with Bcl-2 to suppress programmed cell death pathways. Related references: PMID:10597216
• It is involved in the nucleocytoplasmic shuttling of heat shock factor 1 (HSF1), thereby regulating the cellular stress response. Related references: PMID:26159920
• BAG3 localizes predominantly to the cytoplasm but can also be found in the nucleus under certain conditions.
• Post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination, as identified through proteomics.
• Mutations in BAG3 are associated with cardiomyopathies, myofibrillar myopathy, and Charcot-Marie-Tooth disease, underscoring its critical role in muscle maintenance and neuronal health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 50–150, covering a region that may be conserved in relevant isoforms; verify sequence homology before use in non-human samples.
• For western blotting (WB), a band at approximately 62 kDa is expected; optimize loading controls and consider using 5–10% SDS-PAGE gels for best resolution.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0, heat-induced epitope retrieval) should be tested; the antibody is known to react with human and mouse tissues.
• For ELISA applications, direct or sandwich formats may be employed; consider pairing with a validated capture antibody if designing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for diagnostic-grade raw materials, from concept to clinic. For chaperone-mediated autophagy, apoptosis, and cell stress research, we offer validated anti-BAG3 antibodies like this rabbit monoclonal antibody, alongside a comprehensive portfolio of monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials to support your IVD development.