ATP6V1A Rabbit Polyclonal Antibody for WB, ELISA - P38606

Core Product Specifications and Parameters

Biological Background: ATP6V1A Function and Localization

• ATP6V1A encodes the catalytic subunit A of the V1 domain of vacuolar H+-ATPase (V-ATPase), a multisubunit enzyme that couples ATP hydrolysis to proton translocation (PMID:8463241, PMID:22053050). Known alternate names include V-ATPase 69 kDa subunit, vacuolar ATPase isoform VA68, and vacuolar proton pump subunit alpha. Related references: PMID:8463241 PMID:22053050

• The V-ATPase complex is essential for acidifying and maintaining the pH of intracellular compartments, and in certain cell types, it localizes to the plasma membrane to promote extracellular acidification (PMID:32001091). Related references: PMID:32001091

• On the lysosomal membrane, V-ATPase activates mTORC1 by promoting the guanine nucleotide exchange factor activity of the Ragulator complex (PMID:22053050). Related references: PMID:22053050

• Under aerobic conditions, ATP6V1A contributes to intracellular iron homeostasis by triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, which hydroxylate HIF1A and mark it for proteasomal degradation (PMID:28296633). Related references: PMID:28296633

• The protein may play a role in neurite development and synaptic connectivity (PMID:29668857). Related references: PMID:29668857

• ATP6V1A is predominantly cytosolic but also localizes to clathrin-coated vesicles, secretory vesicles, and lysosomal membranes.

• High protein expression is observed in the skin. Post‑translational modifications include acetylation and phosphorylation, and alternative splicing generates multiple isoforms. The gene is associated with diseases such as epilepsy.

• During Rabies virus infection, ATP6V1A facilitates virion uncoating by interacting with viral matrix M proteins.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 500–600 of human ATP6V1A (NP_001681.2). This region may be conserved across species, consistent with the reported mouse cross-reactivity.

• For Western blot, a band migrating near 68 kDa is expected. Validate antibody specificity using knockdown or knockout controls in the relevant cell or tissue system.

• ELISA conditions should be optimized with recombinant ATP6V1A protein or peptide standards, and non‑specific binding may be reduced with appropriate blocking buffers.

• Since V-ATPase is expressed ubiquitously but enriched in skin, human keratinocyte or fibroblast lysates may serve as positive controls.

• If extending to IHC or IF, consider the antibody’s target epitope in a folded protein context and verify performance under native conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions for autophagy, lysosomal biology, and ion transport research. We provide validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. Whether you need bulk polyclonal antibodies like this anti-ATP6V1A, optimized monoclonal alternatives, or support for rare targets, our team ensures consistent quality and reliable supply from concept to clinic.