ATP1A2 Rabbit pAb for WB, ELISA - P50993

Core Product Specifications and Parameters

Biological Background: ATP1A2 Function and Localization

• ATP1A2 encodes the alpha-2 subunit of the Na+/K+-ATPase, a plasma membrane enzyme responsible for maintaining electrochemical gradients of Na+ and K+ across the cell membrane.
• As the catalytic subunit, it hydrolyzes ATP to exchange three intracellular Na+ for two extracellular K+ per cycle, essential for cellular homeostasis and electrical excitability. Related references: PMID:33880529
• Located on the cell membrane.
• It belongs to the cation transport ATPase (P-type) family, featuring ATP-binding and phosphorylation domains, and requires Mg2+ for activity.
• Associated with diseases such as familial hemiplegic migraine type 2 (FHM2), epilepsy, and intellectual disability, highlighting its critical role in neural function.
• Contains multiple transmembrane helices, forming the ion translocation pathway.
• Undergoes E1-E2 conformational transitions that alternately expose ion-binding sites to the cytoplasm and extracellular side.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1-70 amino acids, which may be exposed on the cytoplasmic side; consider this when designing epitope-based assays.
• For WB, the predicted molecular weight is ~112 kDa; use reducing conditions and validate detection in relevant human, mouse, or rat samples.
• In ELISA, consider coating with the recombinant protein or native antigen; optimize blocking and detection steps for polyclonal antibodies.
• Verify cross-reactivity consistency in your specific sample types and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research labs with streamlined access to validated IVD raw materials, including this ATP1A2 polyclonal antibody for sodium pump and neurological disorder research. Our supply network spans optimized monoclonal/polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents, supported by technical guidance from concept to clinic. Contact us to request this antibody or to source rare-target raw materials tailored to your project needs.