ATMIN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43313

Core Product Specifications and Parameters

Biological Background: ATMIN Function and Localization

• ATMIN (ATM interactor), also known as ASCIZ or ZNF822, is a zinc-finger transcription factor encoded by the ATMIN gene in humans.
• It plays a critical role in cell survival and the formation of RAD51 foci in response to methylating DNA damage, thus involved in DNA repair.
• ATMIN may regulate the activity of the ATM kinase in the absence of DNA damage, potentially stabilizing the ATM protein.
• It binds to the DYNLL1 promoter and activates its transcription, implicating it in transcriptional regulation.
• The protein localizes to the nucleus, consistent with its role as a transcription factor.
• Ubiquitously expressed in normal tissues and cancer cell lines, with highest levels observed in placenta and skeletal muscle.
• Contains zinc-finger domains, suggesting direct DNA binding and transcriptional regulatory functions.
• Alternative splicing generates multiple isoforms, which may have tissue-specific or functional variations.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to amino acids 160-360 of human ATMIN; epitopes may be shared among isoforms. Validate in the relevant sample system.
• For Western blotting, a band around 88 kDa is expected. Use molecular weight markers and appropriate positive/negative controls to confirm specificity.
• For immunofluorescence/ICC, anticipate nuclear staining. Permeabilization may be necessary for intracellular detection.
• Cross-reactivity with mouse and rat is reported, but additional validation in these species is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for diagnostic and research applications. This ATMIN antibody falls within our transcription and DNA damage response portfolio, supporting studies on cancer and cellular stress. Leverage our reliable sourcing capabilities to accelerate your assay development with consistent, batch-to-batch reproducible products.