ATF6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18850

Core Product Specifications and Parameters

Biological Background: ATF6 Function and Localization

• ATF6 is a transcription factor precursor embedded in the endoplasmic reticulum (ER) membrane. ER stress triggers its proteolytic processing, releasing the active N-terminal fragment that translocates to the nucleus to initiate the unfolded protein response (UPR). Related references: PMID:10564271 PMID:11158310 PMID:11779464
• Processed ATF6 binds to ER stress response elements (ERSE, 5'-CCAAT-N₉-CCAC[GA]-3' and ERSE II, 5'-ATTGG-N-CCACG-3') as a heterodimer with NF-Y, activating UPR target genes. Related references: PMID:10564271 PMID:11158310 PMID:11779464 PMID:11163209
• ATF6 may also regulate serum response factor-mediated transcription and plays a role in retinal foveal development and cone function. Related references: PMID:10564271 PMID:11158310 PMID:11779464 PMID:26029869
• Subcellular localization: The precursor resides in the ER and Golgi apparatus membranes; upon processing, the transcription factor domain localizes to the nucleus.
• ATF6 is ubiquitously expressed across human tissues.
• The precursor is an N-linked glycoprotein and can be modified by ubiquitination. The protein is a single-pass type II membrane protein (signal-anchor) with its N-terminus facing the cytoplasm and C-terminus in the ER lumen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 600-670 of human ATF6 (NP_031374.2), located in the C-terminal luminal domain. This region is present in the full-length precursor (~75 kDa). Detection of the cleaved nuclear form (~50 kDa) may vary depending on epitope accessibility; validate in appropriate ER stress models.
• For Western blot, a band near 75 kDa is expected for the precursor. Treat cells with ER stress inducers (e.g., tunicamycin, thapsigargin) to observe processing and nuclear translocation.
• For IHC-P, consider antigen retrieval with citrate or EDTA buffer, and confirm signal specificity with adequate controls.
• In IF/ICC, ATF6 shows a reticular ER/Golgi pattern under basal conditions and redistributes to the nucleus after stress induction; use appropriate fixation and permeabilization.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material supplier, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For researchers investigating ER stress and the unfolded protein response, our ATF6 antibody provides a reliable tool, backed by our commitment to seamless procurement from concept to clinic.