ATF1 Rabbit Polyclonal Antibody for WB, IP, ELISA - P18846

Core Product Specifications and Parameters

Biological Background: ATF-1 Function and Localization

• ATF-1 (Activating Transcription Factor 1) is a cyclic AMP-dependent transcription factor encoded by the ATF1 gene, belonging to the CREB/ATF family of basic leucine zipper (bZIP) transcription factors.
• It binds the cAMP response element (CRE; consensus 5'-GTGACGT[AC][AG]-3') found in many viral and cellular promoters, and also the Tax-responsive element (TRE) of HTLV-I.
• ATF-1 mediates protein kinase A (PKA)-induced stimulation of CRE-reporter genes, linking cAMP signaling to gene expression.
• It acts as a transcriptional repressor of antioxidant detoxification genes such as FTH1, implicating it in oxidative stress responses.
• ATF-1 triggers cell proliferation and transformation, and is involved in chromosomal rearrangements in malignancies (e.g., EWS-ATF1 and FUS/ATF-1 fusions).
• The protein is localized exclusively in the nucleus, consistent with its role as a transcription factor.
• Post-translational modifications include phosphorylation (phosphoprotein) and ubiquitination (Ubl conjugation), and it undergoes alternative splicing.
• Uniprot keywords highlight its functions: DNA-binding, activator, transcription regulation, and its involvement in chromosomal rearrangement.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein covering amino acids 1-210 of human ATF1, which includes the N-terminal region. This region is well-conserved and may be suitable for detecting total ATF-1 protein, but cross-reactivity with other closely related family members (like CREB) should be evaluated by sequence alignment.
• For Western blotting (WB), the predicted molecular weight is 29 kDa; consider using appropriate gel percentages and loading controls. Validate specificity using relevant cell lines (e.g., HeLa, HEK293) and mouse tissues.
• For immunoprecipitation (IP), ensure antibody-antigen complex stability; test various lysis buffer conditions to maintain protein interactions.
• In ELISA, using a peptide or protein standard may help quantify ATF-1 levels; check for linearity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, from concept to clinic. This ATF1 rabbit polyclonal antibody supports researchers developing assays for transcription factor analysis and cancer diagnostics. Beyond single reagents, CamelBio offers validated antibody pairs, optimized bulk antibodies, and ancillary reagents to accelerate your IVD development.