ATE1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O95260

Core Product Specifications and Parameters

Biological Background: ATE1 Function and Localization

• ATE1 encodes arginyl-tRNA--protein transferase 1, an enzyme mediating N-terminal protein arginylation.
• It specifically attaches arginine to the N-terminal aspartate or glutamate residues of acceptor proteins. Related references: PMID:34893540
• This post-translational modification acts as a signal for ubiquitin-dependent proteasomal degradation. Related references: PMID:34893540
• ATE1 does not modify N-terminal cysteine residues (by similarity).
• The protein is found in both the nucleus and cytoplasm, suggesting roles in multiple subcellular compartments.
• ATE1 undergoes alternative splicing and is phosphorylated, indicating regulatory complexity.
• The calculated molecular weight of human ATE1 is approximately 59 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human ATE1, which may be useful for detecting both full-length and N-terminal fragments.
• For Western blot, consider using standard denaturing and reducing conditions; the predicted molecular weight is ~59 kDa.
• In IHC-P, antigen retrieval methods may be required; validate in relevant tissue samples.
• For ELISA, the antibody can be used as a capture or detection reagent after appropriate titration.
• Cross-reactivity with mouse and rat ATE1 suggests conservation of the epitope; verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers validated rabbit monoclonal antibodies for ATE1 detection, supporting N-terminal arginylation and protein degradation studies. As a one-stop IVD raw material supplier, we provide matched antibody pairs, ancillary reagents, and custom sourcing to streamline diagnostic development from concept to clinic.