Asymmetric DiMethyl-Histone H3-R26 (Q16695 / P68431) Rabbit pAb for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, which wraps and compacts DNA into chromatin. This structure limits DNA accessibility to the cellular machineries that require DNA as a template, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications (PTMs) of histones, collectively referred to as the "histone code," as well as through nucleosome remodeling.

Canonical histone H3.1, encoded by multiple genes including H3C1, H3C2, H3C3, etc., is widely expressed. In contrast, histone H3.1t (also known as H3-4 or HIST3H3) is specifically expressed in testicular cells.

At the subcellular level, histone H3 localizes primarily to the nucleus and to chromosomes.

Histone H3 undergoes extensive and varied PTMs, including acetylation, methylation, phosphorylation, citrullination, and others. Arginine residues can be methylated to form asymmetric dimethylarginine (ADMA) by protein arginine methyltransferases (PRMTs) such as PRMT6 and CARM1, which act on specific residues (e.g., R3, R18) and modulate chromatin structure and gene expression. This antibody is designed to specifically recognize asymmetric dimethylation at arginine 26 (R26) of histone H3.

Related references: PMID:17898714, PMID:18077460, PMID:15345777, PMID:15471871

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide containing asymmetric dimethylated R26, designed to confer high specificity for this modification. Researchers should consider verifying specificity using peptide competition assays and by comparing signals with unmodified or differently modified peptides.
• For Western blotting, a specific band at approximately 15 kDa is expected. Histone-enriched extracts or acid-extracted proteins may improve detection. Positive controls derived from cells treated with histone deacetylase inhibitors (e.g., sodium butyrate) can help confirm reactivity.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimal permeabilization and fixation conditions should be determined. Given the predicted wide cross-reactivity, validation in the specific species and tissue of interest is recommended.
• For ELISA applications, use the immunizing peptide as a positive control and a non-modified peptide as a negative control to confirm modification-specific binding.
• As with all post-translational modification-specific antibodies, lot-to-lot consistency and reaction conditions should be monitored in the context of the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes with high-quality antibodies and reagents. This anti-asymmetric dimethyl-Histone H3-R26 product is tailored for epigenetic and chromatin research applications. In addition to individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets. Whether you are developing new assays or scaling up production, we provide reliable supply solutions from concept to clinic.