ASS1 Rabbit pAb - P00966

Core Product Specifications and Parameters

Biological Background: ASS1 Function and Localization

• Encodes argininosuccinate synthase, a key enzyme of the urea cycle that catalyzes the formation of argininosuccinate from aspartate, citrulline and ATP; together with ASL, it is responsible for arginine biosynthesis in most body tissues.
• Belongs to the urea cycle, transforming neurotoxic ammonia into non-toxic urea in the liver of ureotelic animals; plays a critical role in the ammonia detoxification pathway.
• Functionally annotated with several keywords including ATP-binding, ligase activity, and nucleotide-binding; also subject to post-translational modifications such as acetylation and phosphorylation.
• Predominantly expressed in adult liver, consistent with its role in the hepatic urea cycle; subcellularly localized to the cytoplasm/cytosol.
• Also known as citrulline--aspartate ligase (ASS; CTLN1); mutations in ASS1 are associated with citrullinemia type I, a urea cycle disorder.
• 3D structure available; identified in reference proteome and proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human ASS1 (aa 1-412), which may recognize native protein conformations; consider appropriate positive controls such as liver lysates.
• For WB, the expected molecular weight is approximately 47 kDa; validate band specificity in the relevant sample system.
• In IF/ICC, optimize fixation and permeabilization conditions to preserve antigenicity.
• For ELISA, ensure specificity by comparing with a suitable negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This ASS1 antibody supports research into urea cycle disorders and arginine metabolism. We also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing to accelerate your assay development.