ASMT Rabbit Polyclonal Antibody for WB, ELISA - P46597

Core Product Specifications and Parameters

Biological Background: ASMT Function and Localization

• ASMT (Acetylserotonin O-methyltransferase), also known as Hydroxyindole O-methyltransferase (HIOMT), is the key enzyme in melatonin biosynthesis.
• Catalyzes the transfer of a methyl group from S-adenosyl-L-methionine onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).
• Some studies report that ASMT does not exhibit detectable Acetylserotonin O-methyltransferase activity under certain conditions, indicating potential regulatory or isoform-specific variations.
• Encoded by the ASMT gene, which undergoes alternative splicing, generating multiple isoforms with potential functional differences.
• Predominantly expressed in the pineal gland at the protein level, consistent with its role in melatonin production; very low mRNA expression is detected in the retina, but no protein is detected there. Related references: PMID:7989373, PMID:8574683
• Belongs to the methyltransferase family, utilizing S-adenosyl-L-methionine as a cofactor; involved in lipid metabolism through melatonin synthesis.
• The three-dimensional structure has been solved, providing insights into substrate binding and catalytic mechanism.
• Identified in proteomics studies as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 76-298) of human ASMT, which may recognize multiple isoforms if they share this sequence.
• For Western blot, the predicted molecular weight of full-length ASMT is 38 kDa, but consider possible post-translational modifications or isoform variations that could shift the band.
• ELISA conditions should be optimized and validated using the appropriate antigen and detection system in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers a comprehensive suite of raw materials and services, from concept to clinic. For your melatonin biosynthesis research, we provide validated antibody pairs, optimized polyclonal antibodies like this ASMT product, and bulk ancillary reagents. Our team can also assist with sourcing rare targets to accelerate your diagnostic development.