ASC/TMS1 Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - Q9EPB4

Core Product Specifications and Parameters

Biological Background: ASC/TMS1 Function and Localization

• ASC/TMS1, encoded by the Pycard (Asc) gene, is a 21 kDa PYD and CARD domain-containing adaptor protein that mediates apoptosis and inflammation.
  Related references: PMID:32424362 PMID:34678144
• It serves as an integral adapter for assembling NLRP2, NLRP3, NLRP6, and AIM2 inflammasomes, recruiting caspase-1 to process pro-inflammatory cytokines and drive macrophage pyroptosis.
  Related references: PMID:15190255 PMID:15507117 PMID:21892172
• ASC promotes caspase‑8‑ and caspase‑9‑mediated apoptosis by activating the mitochondrial pathway, including BID cleavage and BAX mitochondrial translocation.
• The protein localizes to the cytoplasm, inflammasomes, endoplasmic reticulum, mitochondria, and nucleus, with its nuclear form implicated in inflammasome‑independent transcriptional regulation of cytokines.
• ASC undergoes phosphorylation and ubiquitin‑like conjugation, and is annotated as a phosphoprotein and ubiquitin‑conjugated protein.
• It is broadly expressed across tissues, including small intestine, colon, thymus, spleen, brain, heart, skeletal muscle, kidney, lung, and liver.
• ASC restrains DNA virus infection by cleaving and inactivating the cytosolic DNA sensor cGAS.
  Related references: PMID:28314590

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–93, encompassing the N-terminal PYD domain; a recombinant full-length ASC/TMS1 protein may serve as a positive control in Western blotting.
• For immunofluorescence and IHC, consider using inflammasome-rich tissues such as spleen or colon, and optimize antigen retrieval protocols for formalin-fixed paraffin-embedded samples.
• Validate antibody specificity using ASC/TMS1 knockout or knockdown cell lines, as the protein shares high sequence similarity across human, mouse, and rat.
• In inflammasome activation studies, co-immunostaining with NLRP3 or caspase-1 can help confirm ASC speck formation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement channel for IVD manufacturers and researchers, offering high-quality antibodies like the ASC/TMS1 Rabbit mAb to support inflammasome and apoptosis studies. Beyond this catalog antibody, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare-target raw materials, helping you accelerate diagnostic development from concept to clinic.