AscL1 Rabbit Polyclonal Antibody for WB and ELISA - P50553

Core Product Specifications and Parameters

Biological Background: ASCL1 Function and Localization

• ASCL1 (Achaete-scute homolog 1), also known as ASH1, HASH1, MASH1, and bHLHa46, is a member of the basic helix-loop-helix (bHLH) family of transcription factors.
• It functions as a pioneer transcription factor critical for neuronal differentiation, accessing closed chromatin to enable other factors to bind and activate neural gene expression programs.
• ASCL1 directly binds the E box motif (5'-CANNTG-3') on promoters to promote transcription of neuronal genes.
• Together with POU3F2/BRN2 and MYT1L, ASCL1 is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro.
• It plays essential roles at early stages of development of specific neural lineages in the central nervous system (CNS) and peripheral nervous system (PNS), including generation of olfactory and autonomic neurons.
• ASCL1 acts synergistically with FOXN4 to specify V2b neuron identity over V2a from bipotential p2 progenitors during spinal cord neurogenesis, likely via DLL4-NOTCH signaling.
• The protein localizes to the nucleus, consistent with its role as a transcription factor, and is subject to post-translational modifications such as acetylation (as indicated by UniProt keywords).
• ASCL1 is implicated in neuroendocrine cell development in the glandular stomach and has been identified in proteomics studies; mutations are associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150–236 of human ASCL1, encompassing the conserved bHLH domain critical for DNA binding and dimerization. This design targets a functionally conserved region, likely contributing to the observed cross-reactivity with mouse and rat.
• For Western blot (WB), the predicted molecular weight is approximately 25 kDa (based on UniProt data), but consider potential post-translational modifications or isoform-specific migration shifts in your sample.
• Validate the antibody in your specific sample system, including appropriate positive (e.g., neural tissue or cell lines with neural differentiation) and negative controls, especially given the polyclonal nature.
• For ELISA, note that the antibody is raised against a recombinant protein; consider using a standard curve with the immunogen or a recombinant target protein for quantitative analysis.
• Since ASCL1 is a nuclear transcription factor, sample preparation for WB should include nuclear extraction or efficient lysis to ensure adequate protein recovery.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This polyclonal antibody targeting ASCL1 supports research into neuronal differentiation and neural development. Our supply capabilities extend to validated antibody pairs, optimized recombinant antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD assay development.