ASB17 Rabbit Polyclonal Antibody for WB and ELISA - Q8WXJ9

Core Product Specifications and Parameters

Biological Background: ASB17 Function and Localization

• ASB17 belongs to the ankyrin repeat and SOCS box (ASB) protein family, characterized by an N-terminal ankyrin repeat domain and a C-terminal SOCS box domain.
• It functions as a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
• ASB17 is specifically expressed in the testis and is not detected in other tissues, suggesting a specialized role in testicular function or spermatogenesis.
• The ankyrin repeat domain facilitates protein-protein interactions, while the SOCS box recruits the Elongin BC complex, linking substrates to the ubiquitination machinery.
• Its involvement in the ubiquitin-proteasome pathway marks it as a participant in key cellular processes such as protein quality control and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30-130 of human ASB17; consider epitope accessibility when selecting lysis and detection conditions.
• For Western blot, testis lysates are recommended due to high tissue-specific expression; validate specificity with a negative control tissue.
• In ELISA, the antibody can be used to detect recombinant ASB17; optimize coating antigen concentration and antibody dilution for your assay.
• Cross-reactivity with mouse and rat is reported; verify performance in your species of interest using appropriate samples and assay conditions.
• As a polyclonal antibody, batch-to-batch variability may occur; include internal reference standards in quantitative analyses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, offering validated antibodies like anti-ASB17 for research in ubiquitin-proteasome pathways and testicular biology. Our broad supply capabilities encompass bulk ancillary reagents and rare target sourcing, supporting diagnostic manufacturers and laboratories from concept to clinic. Partner with CamelBio to streamline your raw material procurement and accelerate development timelines.