Aromatase (CYP19A1) Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P11511

Core Product Specifications and Parameters

Biological Background: Aromatase (CYP19A1) Function and Localization

• Aromatase (CYP19A1) is a cytochrome P450 monooxygenase, also known as CYPXIX, Cytochrome P-450AROM, or Estrogen synthase, that catalyzes the conversion of C19 androgens (androstenedione and testosterone) to C18 estrogens (estrone and estradiol). Related references: PMID:27702664, PMID:2848247
• The enzyme carries out three successive oxidations: two conventional oxidations at C19 followed by an aromatization step that cleaves the C10-C19 bond, forming a phenolic A ring and formic acid. Related references: PMID:20385561
• Alternatively, the third oxidation can yield a 19-norsteroid and formic acid, and the enzyme also converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone, potentially contributing to androgen homeostasis. Related references: PMID:22773874
• Aromatase displays 2-hydroxylase activity toward estrone. Related references: PMID:22773874
• Subcellular localization: Endoplasmic reticulum membrane and microsome membrane.
• Tissue specificity: Widely expressed in adult and fetal brain, placenta, skin fibroblasts, adipose tissue, and gonads.
• Key keywords: Heme, iron, metal-binding, transmembrane helix. Disease variants are associated with pseudohermaphroditism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa1-180) of Aromatase; the antibody may recognize the full-length protein and possibly some splice variants containing this region. Validate in your specific sample system.
• For Western blot, expect a band at approximately 58 kDa. Consider optimizing blocking conditions and antibody dilution for your sample type.
• For IHC-P and IF/ICC, appropriate antigen retrieval and permeabilization steps may be required.
• Cross-reactivity with rat has been observed; validate signal specificity in rat tissues if used in multi-species experiments.
• As Aromatase is localized to the endoplasmic reticulum membrane, ensure proper membrane protein extraction protocols for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom monoclonal/polyclonal antibody development. This Aromatase (CYP19A1) antibody is part of our hormone signaling and oncology research catalog, designed to support your assay development from concept to clinic. Contact us to discuss rare target sourcing and end-to-end workflow solutions.