ARHGDIB Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P52566

Core Product Specifications and Parameters

Biological Background: ARHGDIB Function and Localization

• ARHGDIB, also known as RhoGDI2, Ly-GDI, Rho-GDI beta, D4, GDIA2, GDID4, LYGDI, or RAP1GN1, is a member of the Rho GDP-dissociation inhibitor family.
• It tightly regulates the GDP/GTP exchange reaction of Rho proteins by inhibiting the dissociation of GDP from them and the subsequent binding of GTP. Related references: PMID:7512369 PMID:8356058
• ARHGDIB participates in the regulation of actin cytoskeleton reorganization mediated by Rho family members. Related references: PMID:8262133
• The protein is predominantly localized in the cytoplasm, specifically the cytosol.
• Expression is primarily detected in hematopoietic tissues, including bone marrow, thymus, and spleen.
• The calculated molecular weight is approximately 23 kDa, consistent with the UniProt entry (22,988 Da).
• Key post-translational modifications include acetylation and phosphorylation; the protein has been characterized by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein covering the full-length human ARHGDIB (amino acids 1–201). It may recognize multiple epitopes, offering robust detection across assays.
• For Western blot (WB), consider probing lysates from human or mouse hematopoietic cell lines or tissues (e.g., bone marrow, spleen) where ARHGDIB is enriched. Validate with appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), tissue sections from bone marrow, thymus, or spleen are recommended candidates. Antigen retrieval and antibody dilution should be empirically optimized.
• In ELISA, use the antibody as a capture or detection reagent, noting that polyclonal reactivity can enhance sensitivity but may require stringent blocking.
• Cross-reactivity has been confirmed with human and mouse, but performance in other species should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material procurement, offering validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents to support your assay development. With expertise in targets such as RhoGDI2, we provide tailored sourcing solutions for rare and essential proteins involved in Rho GTPase signaling and cytoskeletal dynamics. Accelerate your diagnostic pipeline with CamelBio's comprehensive supply chain—from research-grade materials to scalable production.