ARHGAP30 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7Z6I6

Core Product Specifications and Parameters

Biological Background: ARHGAP30 Function and Localization

• ARHGAP30 encodes Rho GTPase-activating protein 30, also known as Rho-type GTPase-activating protein 30.
• It functions as a GTPase-activating protein (GAP) for the small GTPases RAC1 and RHOA, accelerating their intrinsic GTP hydrolysis, but does not exhibit GAP activity toward CDC42.
• The protein localizes to cytoplasmic vesicles, as indicated by subcellular fractionation and immunofluorescence studies.
• ARHGAP30 is subject to phosphorylation, as identified in phosphoproteomic screens, suggesting regulation by kinase signaling pathways.
• Alternative splicing generates multiple transcript variants, potentially expanding the functional diversity of this protein.
• It is classified under the GTPase activation keyword and has been detected in proteomics identification studies, validating its expression at the protein level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 485-585) of human ARHGAP30, which may enable recognition of multiple splice isoforms; verify specificity in your experimental system.
• For Western blot, the predicted molecular weight is approximately 119 kDa; use appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC, expect a cytoplasmic vesicular staining pattern consistent with its subcellular location; include appropriate organelle markers for co-localization if desired.
• This antibody has been validated in human and mouse samples; when working with other species, confirm cross-reactivity empirically.
• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; always titrate to determine the optimal working dilution for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with IVD manufacturers and research labs to supply high-quality raw materials for immunoassay development, including validated antibodies like this ARHGAP30 polyclonal. Our portfolio covers key signaling targets, and we facilitate access to bulk ancillary reagents and rare-target sourcing to streamline your R&D pipeline. Whether you need assay-ready antibody pairs or customized raw-material solutions, CamelBio serves as your integrated sourcing bridge from concept to clinic.