Arginase-1 Rabbit mAb for WB, IF-P, IHC-P, ELISA - Q61176

Core Product Specifications and Parameters

Biological Background: Arginase-1 Function and Localization

• Arginase-1 (Arg-1), also known as Liver-type arginase or Type I arginase, is a key enzyme in the urea cycle that converts L-arginine to urea and L-ornithine, playing a central role in nitrogen detoxification and the biosynthesis of proline and polyamines.
• It regulates L-arginine availability for nitric oxide synthase (NOS) in nonhepatic tissues, thereby modulating innate and adaptive immune responses, including T cell and NK cell function.
• In group 2 innate lymphoid cells (ILC2s), Arginase-1 promotes acute type 2 inflammation and is required for optimal ILC2 proliferation (PubMed:27043409). Related references: PMID:27043409
• In alternatively activated (M2) macrophages, it supports wound healing, tissue regeneration, defense against parasites, and immunosuppression, with organ-specific outcomes (PubMed:19360123, PubMed:20483789, PubMed:23552798). Related references: PMID:19360123, PMID:20483789, PMID:23552798
• In tumor microenvironments, Arginase-1 expressed by dendritic cells and myeloid-derived suppressor cells (MDSCs) suppresses T cell-mediated antitumor immunity (PubMed:19414774, PubMed:23248265). Related references: PMID:19414774, PMID:23248265
• Subcellularly, it localizes to the cytoplasm and cytoplasmic granules. It is expressed in macrophages, ILC2s, lung tumor-associated myeloid cells, and tumor-infiltrating dendritic cells.
• As a phosphoprotein and manganese-dependent hydrolase, Arginase-1 requires metal binding for catalytic activity and is involved in both innate and adaptive immunity.

Experimental Guidance and Technical Tips

• Based on the immunogen design (N-terminal 1-100 amino acids), this antibody may recognize the native protein in various applications.
• For Western blot (WB), a band around 35 kDa is expected; consider using appropriate positive controls such as liver or macrophage lysates.
• In IHC-P and IF-P, optimize antigen retrieval and permeabilization steps as the target is cytoplasmic.
• For ELISA, validate using recombinant Arginase-1 or biological samples containing the native enzyme.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows. This anti-Arginase-1 rabbit monoclonal antibody is suitable for studies on amino acid metabolism, immune regulation, and oncology. For specialized requirements, CamelBio's sourcing network can help secure rare-target raw materials and custom formulations to accelerate your assay development.