AQP5 Rabbit pAb for WB, IHC-P, ELISA - P55064

Core Product Specifications and Parameters

Biological Background: AQP5 Function and Localization

• Aquaporin-5 (AQP5) is a water-specific channel protein belonging to the aquaporin family, encoded by the AQP5 gene.
• It forms homotetrameric transmembrane channels, with each monomer independently transporting water across the plasma membrane along its osmotic gradient. Related references: PMID:18768791, PMID:8621489
• AQP5 is essential for fluid secretion in salivary glands and works with TRPV4 to regulate hypotonicity-induced cell volume decrease. Related references: PMID:16571723
• It appears to play redundant roles in water transport in the eye, lung, and sweat glands (By similarity).
• AQP5 localizes to the apical cell membrane, cell membrane, and cytoplasmic vesicle membrane.
• Clinically, AQP5 mutations are associated with palmoplantar keratoderma, and the protein is subject to glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 200–265). Consider this specificity when designing experiments.
• For Western blot (WB), expect a band near 28 kDa. Validate in relevant sample systems using appropriate positive controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and test on tissues such as salivary gland or skin, where AQP5 is expressed.
• In ELISA, use the antibody in a validated assay format; consider pairing with other AQP5-specific reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibodies, antigen pairs, and ancillary reagents to accelerate diagnostic development. For aquaporin research and water transport studies, we provide optimized AQP5 polyclonal antibodies and can source rare-target raw materials to meet your project needs from concept to clinic.