Apolipoprotein CI Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P02654

Core Product Specifications and Parameters

Biological Background: Apolipoprotein CI (APOC1) Function and Localization

• Apolipoprotein C-I (APOC1) is a key component of very low density lipoproteins (VLDL) and high density lipoproteins (HDL), constituting about 10% of VLDL protein and 2% of HDL protein.
• It acts as a potent inhibitor of lipoprotein binding to the LDL receptor, LDL receptor-related protein (LRP), and VLDL receptor, regulating lipid uptake by cells.
• APOC1 directly interferes with cellular fatty acid uptake and reduces the intracellular esterification of free fatty acids.
• It serves as the major plasma inhibitor of cholesteryl ester transfer protein (CETP), influencing the exchange of cholesteryl esters between lipoproteins.
• The protein modulates the interaction of apolipoprotein E (APOE) with beta-migrating VLDL and inhibits the binding of beta-VLDL to LRP.
• APOC1 is a secreted protein primarily synthesized in the liver, with low-level expression in the intestine, lung, and spleen.
• It binds free fatty acids and is annotated with keywords including 3D-structure, direct protein sequencing, lipid transport, secreted, and VLDL, reflecting its role in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human APOC1; confirm sequence homology with target species if cross-reactivity is critical.
• For Western blotting, anticipate a band around 9 kDa; consider optimizing transfer conditions for such low molecular weight proteins.
• Since APOC1 is secreted, appropriate sample preparation (e.g., plasma, serum, or conditioned medium) may be needed for immunodetection.
• Validate the antibody in your specific assay system using recommended positive controls (e.g., human liver lysates or plasma).
• Titrate the antibody for each application (WB, IF-P, IHC-P, ELISA) to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw material sourcing, offering this Apolipoprotein CI monoclonal antibody to support lipid metabolism and cardiovascular research. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized procurement of rare targets. Contact us for flexible supply solutions that help streamline your assay development from concept to clinic.