APEX1/APE1 Rabbit mAb for WB, IHC-P, ELISA - P27695

Core Product Specifications and Parameters

Biological Background: APEX1/APE1 Function and Localization

• Multifunctional protein central to oxidative stress response; possesses DNA repair and redox regulatory activities (PubMed:11118054, PubMed:11452037, PubMed:15831793). Related references: PMID:11118054, PMID:11452037, PMID:15831793
• Functions as an AP endonuclease in base excision repair, incising the phosphodiester backbone at abasic sites; also active on DNA/RNA hybrids and single-stranded substrates (PubMed:15380100, PubMed:16617147, PubMed:18439621). Related references: PMID:15380100, PMID:16617147, PMID:18439621
• Exhibits 3'-5' exodeoxyribonuclease and DNA 3' phosphodiesterase activities to process blocked DNA ends during short-patch BER (PubMed:11832948, PubMed:1719477, PubMed:7516064). Related references: PMID:11832948, PMID:1719477, PMID:7516064
• Acts as an endoribonuclease controlling single-stranded RNA metabolism, cleaving MYC mRNA and participating in rRNA quality control (PubMed:19188445, PubMed:19401441, PubMed:21762700). Related references: PMID:19188445, PMID:19401441, PMID:21762700
• Redox regulates transcription factor DNA binding, e.g., FOS/JUN AP-1 complex, and mediates calcium-dependent PTH repression (PubMed:10023679, PubMed:8621488, PubMed:11809897, PubMed:14633989). Related references: PMID:10023679, PMID:8621488, PMID:11809897, PMID:14633989
• Acetylated at Lys-6/Lys-7; stimulates YBX1-mediated MDR1 promoter activity, contributing to drug resistance (PubMed:18809583). Related references: PMID:18809583
• Localizes to nucleus, nucleolus, speckles, cytoplasm, ER, and mitochondria, reflecting diverse functions.
• Interacts with NPM1 for rRNA association and with XRCC5/XRCC6 for transcriptional repression (PubMed:19188445, PubMed:19401441, PubMed:20699270). Related references: PMID:19188445, PMID:19401441, PMID:20699270

Experimental Guidance and Technical Tips

• This antibody was raised against an N-terminal synthetic peptide (aa 1–100), allowing recognition of full-length APEX1/APE1.
• For Western blotting, a band at approximately 36 kDa is expected. Validate in the relevant sample system and optimize loading and transfer conditions.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods and include appropriate positive and negative tissue controls.
• For ELISA, the antibody can be utilized as a capture or detection reagent; pair with a compatible secondary antibody and confirm specificity.
• Cross-reactivity is reported with human, mouse, and rat; verify in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering antibodies like this anti-APEX1/APE1 mAb for DNA repair and oxidative stress research. We provide validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to support your diagnostic development from concept to clinic.