APC/Cyanine7 Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for FC - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• H-2 class II histocompatibility antigen, I-E beta chain (gene H2-Eb1) is a component of MHC class II molecules, which present peptide antigens to CD4+ T lymphocytes, initiating adaptive immune responses.
• The protein is a single-pass type I membrane protein localized on the cell surface of antigen-presenting cells.
• It contains characteristic structural features including a signal peptide, extracellular domains with disulfide bonds, a transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation and ubiquitination (Ubl conjugation).
• As part of the MHC class II complex, it plays a critical role in self/non-self discrimination, T-helper cell activation, and antibody production.

Experimental Guidance and Technical Tips

• This antibody was raised against recombinant protein covering mouse MHC Class II (I-A/I-E); it may recognize multiple alleles and haplotypes.
• For flow cytometry, we recommend titrating the antibody to determine the optimal concentration for your specific cell type and staining procedure.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specificity and proper gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic assay development. This APC/Cyanine7-conjugated anti-MHC Class II monoclonal antibody is suitable for immune cell profiling studies and assay design. Our team can assist in sourcing rare-target reagents and provide technical consultancy to support your project from concept to clinical application.