APC/Cyanine7 Rat anti-Mouse CD45R/B220 Monoclonal Antibody for FC - P06800

Core Product Specifications and Parameters

Biological Background: CD45R/B220 (CD45) Function and Localization

• CD45, designated receptor-type tyrosine-protein phosphatase C, is a transmembrane glycoprotein also known as leukocyte common antigen (L-CA), Ly-5, or T200. It serves as a critical regulator of lymphocyte activation and differentiation.
• As a tyrosine-protein phosphatase, CD45 is essential for T-cell activation through the antigen receptor. It positively regulates T-cell coactivation upon binding to DPP4, facilitating signal transduction.
• The intracellular region contains two tandem phosphatase domains. The first domain (D1) possesses catalytic activity, while the second domain (D2) influences the substrate specificity of D1, fine-tuning signal output.
• During T-cell activation, CD45 recruits and dephosphorylates key signaling molecules, including SKAP1 and FYN. It also dephosphorylates LYN, thereby modulating LYN kinase activity and downstream signaling pathways.
• At the interface between antigen-presenting cells and T cells, CD45 interacts with CLEC10A on immature dendritic cells. This interaction, involving Tn antigen-carrying CD45 on effector T cells, modulates the T-cell activation threshold to limit autoreactivity.
• CD45 is localized to the cell membrane, membrane rafts, and immunological synapses, positioning it at the hub of immune signaling. Alternative splicing generates multiple isoforms; the B220 isoform, recognized by this antibody, is predominantly expressed on B lymphocytes and is a classic B-cell lineage marker.
• Keywords: alternative splicing, cell membrane, glycoprotein, hydrolase, membrane, phosphoprotein, protein phosphatase, synapse, transmembrane, transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody is supplied as an APC/Cyanine7 conjugate, optimized for flow cytometry (FC). Verify fluorescence compensations using single-stained controls and include an appropriate isotype control to set gating thresholds.
• The immunogen consists of recombinant mouse CD45R/B220 protein. For optimal staining, titrate the antibody in the relevant biological matrix (e.g., mouse spleen or lymph node cell suspensions) to determine the concentration that yields maximal specific signal with minimal background.
• Consider performing multicolor panel design carefully to avoid spectral overlap; APC/Cyanine7 is typically excited by a 635 nm laser and emits in the far-red channel, requiring compensation with other fluorophores in that range.
• Validate expression in the intended sample system, such as mouse lymphoid tissues, where B220 is highly expressed on B cells. For non-standard applications, pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to empowering IVD manufacturers, research laboratories, and diagnostic companies with streamlined access to premium raw materials. This anti-CD45R/B220 monoclonal antibody exemplifies our commitment to providing quality reagents for immunology and hematology applications. Leverage our one-stop supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets to accelerate your diagnostic development from concept to clinic.