APC/Cyanine7 Rabbit anti-Mouse CD90.2 Monoclonal Antibody for Flow Cytometry - P01831

Core Product Specifications and Parameters

Biological Background: Thy-1 Membrane Glycoprotein Function and Localization

• Thy-1 (CD90) is a GPI-anchored cell surface glycoprotein belonging to the immunoglobulin superfamily, encoded by the Thy1 gene in mouse.
• It is expressed on the outer leaflet of the plasma membrane and serves as a marker for various stem cells and immune cell subsets.
• Functionally, Thy-1 is implicated in cell-cell and cell-matrix interactions, particularly playing a role in synaptogenesis and other neuronal processes in the brain.
• The protein contains a single V-type immunoglobulin domain and is modified by N-linked glycosylation and a disulfide bond, essential for its structural integrity.
• Thy-1 is attached to the membrane via a glycosylphosphatidylinositol (GPI) anchor, which facilitates its localization to lipid rafts and potential involvement in signal transduction.
• Post-translational modifications include formation of a pyrrolidone carboxylic acid at the N-terminus and removal of a signal peptide during processing.
• The canonical isoform has a molecular weight of approximately 18 kDa (18,080 Da as per UniProt), though glycosylation can increase the apparent mass in electrophoresis.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to APC/Cyanine7, making it ready-to-use for flow cytometry without secondary staining. Protect from light during handling and storage to preserve fluorophore integrity.
• Since the immunogen is a synthetic peptide derived from mouse CD90.2, the antibody likely recognizes a linear epitope; denaturing conditions may not be suitable without specific validation.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining protocol. Include appropriate isotype controls and live/dead discrimination.
• Validate the antibody in the relevant mouse model system, as cross-reactivity with rat or human CD90 may not be guaranteed. Mouse-specific expression patterns should be confirmed using known positive (e.g., splenocytes, thymocytes) and negative cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, academic laboratories, and research institutes with streamlined access to premium IVD raw materials, from validated monoclonal antibodies like this APC/Cyanine7-conjugated anti-CD90.2 clone to bulk ancillary reagents and rare target sourcing. Whether you are developing immunological assays or neuroscience tools, our one-stop procurement model supports your entire workflow—from concept to clinic—with consistent quality and technical expertise.