APC/Cyanine7 Rabbit anti-Mouse CD8a Monoclonal Antibody for Flow Cytometry - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• T-cell surface glycoprotein CD8 alpha chain (CD8a) is an integral membrane protein that serves as a coreceptor for MHC class I molecules, playing an essential role in adaptive immunity and recognition of intracellular pathogens.
• CD8a interacts simultaneously with the T-cell receptor (TCR) and MHC class I:peptide complex on antigen-presenting cells, recruiting the Src kinase LCK to initiate signaling cascades that lead to cytotoxic T-cell activation, cytokine production, and target cell killing.
• In natural killer (NK) cells, CD8A homodimers promote conjugate formation and lysis of multiple target cells, and support the differentiation of activated lymphocytes into memory CD8+ T-cells.
• The protein is localized to the cell membrane via a single-pass transmembrane domain and is modified by palmitoylation, glycosylation, and disulfide bond formation within its immunoglobulin-like domain.
• Alternative splicing generates multiple isoforms, and the protein is classified under keywords such as adaptive immunity, disulfide bond, glycoprotein, palmitate, and signal.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions; a starting dilution range of 1:100–1:400 is typical for surface antigens.
• Use appropriate isotype controls and live/dead discrimination to ensure accurate gating; mouse splenocytes or thymocytes serve as ideal positive control tissues for CD8a expression.
• APC/Cyanine7 is a tandem fluorophore; minimize exposure to light, and be aware that fixation and permeabilization steps may alter tandem dye stability and fluorescence intensity.
• Validate antibody binding under your experimental conditions by comparing staining patterns against known biological positive and negative populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including optimized monoclonal antibodies, validated antibody pairs, and ancillary reagents. This APC/Cyanine7 Rabbit anti-Mouse CD8a mAb supports immuno-oncology and immune profiling workflows, and CamelBio can facilitate bulk supply and custom conjugation services to accelerate your assay development from concept to clinic.