APC/Cyanine7 Rabbit anti-Mouse CD34 Monoclonal Antibody for FC - Q64314

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 (Hematopoietic progenitor cell antigen CD34) is a transmembrane glycoprotein belonging to the sialomucin family.
• Functions as a putative adhesion molecule in early hematopoiesis, mediating stem cell attachment to the bone marrow extracellular matrix or directly to stromal cells; may serve as a scaffold for lineage-specific glycans that facilitate lectin binding (By similarity).
• The Cd34 gene undergoes alternative splicing, generating multiple isoforms.
• Localized to the cell membrane as a single-pass type I transmembrane protein.
• Tissue expression: Highly expressed in hematopoietic progenitor cell lines, brain, and testis; moderately in thymus, spleen, and bone marrow; absent in adult liver; also detected in the kidney's thin limb of Henle's loop at the protein level. Related references: PMID:31605441 PMID:1709048
• Presents carbohydrate ligands to selectins, implicating a role in cell trafficking and homing.
• Key post-translational modifications include glycosylation and phosphorylation; features a signal peptide and a transmembrane helix.

Experimental Guidance and Technical Tips

• This APC/Cyanine7-conjugated rabbit monoclonal antibody is designed for flow cytometry applications. Consider titrating the antibody to determine the optimal concentration for your specific cell type and staining protocol.
• Use appropriate isotype controls and viability dyes to ensure specific staining and accurate gating.
• Validate the staining performance in relevant mouse hematopoietic progenitor cell lines, bone marrow, or other Cd34-expressing tissues like testis or brain.
• Avoid multiple freeze-thaw cycles; store the antibody as recommended (ice bag shipping) to maintain conjugate stability and fluorescence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research institutions with a single point of access to high-quality raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. For CD34-related diagnostics and research, our one-stop sourcing covers optimized antibodies, bulk orders, and rare-target raw-material procurement to support your assay development from concept to clinic. Reach out to explore tailored sourcing solutions that fit your workflow.