APC/Cyanine7 Rabbit anti-Mouse CD25 Monoclonal Antibody for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25, also known as Interleukin-2 receptor subunit alpha (IL-2RA) or p55, is encoded by the Il2ra gene (GeneID: 16184) in mice. It is a key component of the high-affinity IL-2 receptor complex.
• CD25 functions as a receptor for interleukin-2, playing a critical role in immune homeostasis and regulation.
• It is essential for controlling regulatory T cell (Treg) activity; Tregs suppress the activation and expansion of autoreactive T cells, thereby maintaining peripheral immune tolerance.
• Subcellular localization is primarily at the plasma membrane as a type I membrane protein with an extracellular ligand-binding domain.
• The protein features characteristic structural motifs including signal peptide, sushi (CCP/SCR) domains, a transmembrane helix, and post-translational modifications such as glycosylation and disulfide bonds.
• UniProt keyword annotations classify CD25 as a disulfide bond-containing glycoprotein receptor with transmembrane topology, involved in immunity.
• The mature protein has a theoretical molecular weight of approximately 30.7 kDa, but glycosylation increases the apparent weight to around 31 kDa as observed in SDS-PAGE.

Experimental Guidance and Technical Tips

• This antibody is supplied as an APC/Cyanine7 conjugate for direct flow cytometry (FC) applications; no secondary antibody is required.
• The immunogen encompasses amino acids 22–236, which corresponds to the extracellular domain, making it suitable for detecting surface CD25 on live cells without permeabilization.
• For optimal results, titrate the antibody to determine the appropriate concentration for your specific cell type and assay conditions; include appropriate isotype controls.
• Consider verifying cross-reactivity in your mouse strain of interest; the antibody is validated for mouse CD25.
• Avoid multiple freeze-thaw cycles and store according to the recommended conditions to maintain conjugate stability and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents from concept to clinic. This CD25 monoclonal antibody supports immune monitoring and autoimmune disease research; CamelBio can further supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development. Whether you need rare target reagents or large-scale production lots, CamelBio offers one-stop access to meet your requirements.