APC/Cyanine7 Rabbit anti-Mouse CD19 mAb for FC - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 is a B-lymphocyte antigen and differentiation marker, functioning as a coreceptor for the B-cell receptor (BCR) complex on B cells.
• It lowers the threshold for BCR activation, potentiating downstream signaling cascades through phosphatidylinositol 3-kinase (PI3K) and intracellular calcium mobilization. Related references: PMID:12387743, PMID:20101619, PMID:9382888
• CD19 is dispensable for early B-cell differentiation in the bone marrow but is essential for normal B-1 cell development and antigen-driven B-cell proliferation/differentiation. Related references: PMID:7542548, PMID:7543183, PMID:9317126, PMID:12387743
• It is required for normal serum immunoglobulin levels and production of high-affinity antibodies in response to antigen challenge. Related references: PMID:12387743, PMID:7542548, PMID:7543183
• CD19 is localized on the cell membrane and enriched in membrane rafts.
• Tissue expression: CD19 is detected on B cells in spleen, bone marrow, thymus, lymph nodes, and peripheral blood lymphocytes. Related references: PMID:12387743, PMID:20101619, PMID:7542548, PMID:7543183
• The protein is a transmembrane glycoprotein with immunoglobulin domains, disulfide bonds, and phosphorylation sites, indicative of roles in signal transduction and immune regulation.

Experimental Guidance and Technical Tips

• This antibody is an APC/Cyanine7-conjugated rabbit monoclonal antibody specific for mouse CD19, recommended for flow cytometry.
• As the immunogen is recombinant mouse CD19 protein, consider verifying specificity using CD19-negative cell lines or isotype controls.
• Determine the optimal working concentration experimentally; protect the conjugate from light to maintain fluorescence.
• Ensure proper sample preparation and staining protocols for best resolution in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we support diagnostic and research laboratories with a comprehensive portfolio of raw materials. This anti-mouse CD19 antibody exemplifies our commitment to providing high-quality B-cell research tools, including validated antibody pairs, optimized conjugates, and bulk ancillary reagents. Partner with us to streamline your IVD development from concept to clinic.