APC/Cyanine7 Rabbit anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M (CD11b) Function and Localization

• Integrin alpha-M (CD11b) is the alpha subunit of the leukocyte-specific integrin ITGAM/ITGB2 (also known as CR3 or MAC-1), a receptor for complement component iC3b, as well as fibrinogen, factor X, and ICAM1.
• ITGAM/ITGB2 mediates adhesion and phagocytosis in monocytes, macrophages, and granulocytes, and regulates neutrophil migration.
• In association with ITGB2 and CD177-PRTN3, it participates in the activation of TNF-primed neutrophils.
• In microglia, ITGAM/ITGB2 is required together with TYROBP/DAP12 to produce superoxide ions that promote developmental neuronal apoptosis. Related references: PMID:18685038
• The protein is localized to the cell membrane and membrane rafts, consistent with its role in cell adhesion and signaling.
• CD11b is predominantly expressed in monocytes and granulocytes, but also found in peritoneal mast cells and microglia.
• It is a glycoprotein with multiple disulfide bonds and calcium-binding sites, which are essential for its integrin function.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for staining mouse leukocytes, as the CD11b antigen is expressed on monocytes, granulocytes, and subsets of mast cells and microglia.
• Since the immunogen corresponds to an extracellular region (amino acids 143-337), this antibody is likely to recognize surface CD11b on live cells without permeabilization; however, validate staining conditions in your own experimental system.
• Ensure adequate compensation for the APC/Cyanine7 tandem dye, which can exhibit degradation and spillover into the APC channel under certain conditions; use fresh conjugate and include appropriate controls.

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