APC/Cyanine7 Rabbit Anti-Human/Monkey IgD Monoclonal Antibody for Flow Cytometry - P01880

Core Product Specifications and Parameters

Biological Background: IgD Function and Localization

• IgD is the constant region of delta heavy chains, existing as both membrane-bound (mIgD) and secreted (sIgD) immunoglobulins, and serves as the primary antigen receptor on peripheral B-cells, usually co-expressed with IgM. Related references: PMID:20176268, PMID:22158414
• Antigen binding to mIgD triggers B-cell clonal expansion and differentiation into plasma cells, a process involving CD79A and CD79B phosphorylation by Src family kinases. Related references: PMID:20176268, PMID:22158414
• Soluble IgD concentration in serum is lower than IgG, IgA, and IgM but higher than IgE, and its secretion is downregulated following B-cell receptor engagement.
• IgD is a potent inducer of pro-inflammatory cytokines (TNF, IL1B, IL1RN) and other cytokines (IL6, IL10, LIF) from peripheral blood monocytes. Related references: PMID:10702483, PMID:11282392, PMID:8774350
• Variable domains of IgD are assembled by V(D)J recombination and undergo somatic hypermutation, allowing affinity maturation for antigens. Related references: PMID:17576170, PMID:20176268
• Multiple isoforms of the IgD heavy chain are produced by alternative splicing; the protein is N-glycosylated and features conserved intrachain and interchain disulfide bonds.
• Subcellular localization includes the plasma membrane (for mIgD) and secretion (for sIgD); the transmembrane form contains a single transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 99–384 of human IgD, which includes a substantial portion of the extracellular region; this design may favor recognition of native surface IgD on B-cells.
• Given the verified reactivity with human and cynomolgus monkey, consider validating performance in relevant peripheral blood or lymphoid tissue samples from these species.
• The APC/Cyanine7 conjugate is compatible with standard red (633–647 nm) laser lines; ensure appropriate filter sets and compensation controls are used in multicolor flow cytometry panels.
• When co-staining with IgM or other B-cell markers, verify that the fixation and permeabilization protocol does not interfere with the extracellular epitope recognized by this clone.
• As with any flow cytometry reagent, titrate the antibody to determine the optimal concentration for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials for B-cell immunophenotyping and humoral immunity research, including the IgD target represented by this antibody. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to support your diagnostic and research workflows. Leverage CamelBio’s technical consultation and logistics expertise to streamline your development from concept to clinic.