APC/Cyanine7 Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for FC - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is an integral membrane glycoprotein also known as T-cell surface antigen T4/Leu-3, encoded by the CD4 gene in humans.
• It functions primarily as a coreceptor for MHC class II molecules on antigen-presenting cells, interacting with the T-cell receptor (TCR) and recruiting the Src kinase LCK to initiate T-cell activation, lymphokine production, and T-helper cell differentiation. Related references: PMID:12089508 PMID:16331979 PMID:2214026
• CD4 also plays roles in macrophages and NK cells, mediating differentiation, cytokine expression, and migration through TCR/LCK-independent pathways, and triggers monocyte-to-macrophage maturation.
• As the primary receptor for HIV-1, CD4 facilitates viral entry, and its down-regulation by HIV-1 Vpu protein contributes to immune evasion; it also serves as a receptor for Human Herpes virus 7 (HHV-7). Related references: PMID:17346169 PMID:7909607
• The protein is localized to the cell membrane and contains key structural features: an extracellular region with immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail with palmitoylation and phosphorylation sites.
• Expression of CD4 is highly enriched on T-helper cells and also present on macrophages, dendritic cells, and NK cells, serving as a hallmark marker for the T-helper lineage.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein of human CD4, so consider that the antibody likely recognizes an extracellular epitope accessible for live-cell staining in flow cytometry.
• For flow cytometry (FC), validate the antibody in the relevant sample system; typical staining protocols for surface CD4 on human or cynomolgus peripheral blood mononuclear cells (PBMCs) may be used, with appropriate isotype controls.
• Due to the APC/Cyanine7 conjugation, be mindful of fixation and permeabilization steps that may affect fluorophore stability; testing in your specific assay is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive IVD raw-material partner, offering a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This APC/Cyanine7 anti-CD4 antibody supports immunophenotyping and T-helper cell research needs. We streamline procurement from discovery to diagnostics, ensuring quality supply for your assay development.