APC/Cyanine7 Rabbit anti-Human/Monkey CD38 PolymAb® - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein that serves as an enzyme (ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase) and a receptor, playing roles in calcium mobilization, signal transduction, and cell adhesion.
• It synthesizes cyclic ADP-ribose (cADPR), a second messenger essential for glucose-induced insulin secretion. Related references: PMID:7961800, PMID:8253715
• CD38 also produces nicotinate-adenine dinucleotide phosphate (NAADP+), a potent Ca²⁺ mobilizer, from NADP⁺ and nicotinic acid, with a pH-dependent preference. Related references: PMID:16690024
• As a receptor, CD38 binds CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and transendothelial migration; in lamina propria T lymphocytes, this interaction triggers LCK and LAT activation and cytokine release. Related references: PMID:9551996, PMID:11259373
• In dendritic cells, CD38 regulates mature functions such as CCL21-driven migration, survival, and Th1-polarizing activity. Related references: PMID:16293598
• CD38 is highly expressed in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma, and neuroblastoma.
• It localizes to the cell surface and cell membrane via a signal-anchor transmembrane helix.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 43–300 of human CD38, covering the extracellular domain, making it suitable for detecting surface-expressed CD38 by flow cytometry.
• This APC/Cyanine7 conjugate is optimized for flow cytometry; consider including appropriate isotype controls, Fc receptor blocking, viability dyes, and dead cell exclusion for accurate gating.
• Validate the antibody in your specific experimental system, particularly when using cynomolgus monkey samples, as cross-reactivity has been confirmed.
• For multicolor panels, check fluorophore compatibility and perform compensation controls to minimize spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing diagnostic manufacturers and researchers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD38, a key marker in immune cell profiling and oncology, we offer this high-quality APC/Cyanine7-conjugated antibody along with rare target raw-material sourcing to accelerate your assay development from concept to clinic.