APC/Cyanine7 Rabbit Anti-Human/Monkey CD25 Monoclonal Antibody for FC - P01589

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25, also known as IL-2 receptor subunit alpha, TAC antigen, and p55, is encoded by the IL2RA gene.
• It serves as the high-affinity alpha chain of the interleukin-2 receptor, critical for IL-2 signaling.
• CD25 plays a pivotal role in immune homeostasis by supporting regulatory T cell (Treg) function, which suppresses autoreactive T cell activation.
• The protein is a type I transmembrane glycoprotein located on the plasma membrane.
• Its extracellular region contains sushi domains and disulfide bonds essential for receptor structure.
• Post-translational modifications include N-linked glycosylation.
• Genetic variants in IL2RA are associated with susceptibility to type 1 diabetes mellitus and other immune dysregulation disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 22-213), suggesting suitability for detecting surface CD25 by flow cytometry.
• For optimal results, titrate the antibody in your experimental system and consider using appropriate isotype controls.
• The antibody cross-reacts with both human and cynomolgus monkey CD25, enabling translational research models.
• Validate fixation and permeabilization compatibility if intracellular staining is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, supporting your CD25-targeted assay development with validated antibody pairs, optimized monoclonal antibodies like this APC/Cyanine7 rabbit anti-CD25 clone, and ancillary reagents. Our one-stop supply model helps diagnostic manufacturers and research labs streamline procurement from concept to clinic, including rare target raw-material sourcing for immune regulation studies.