APC/Cyanine7 Rabbit anti-Human TIM-3/HAVCR2 Monoclonal Antibody for Flow Cytometry - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• TIM-3 (HAVCR2, CD366) is a type I transmembrane glycoprotein and immune checkpoint receptor belonging to the T-cell immunoglobulin and mucin domain (TIM) family.
• Functions primarily as an inhibitory receptor on T-cells, suppressing Th1-mediated auto- and alloimmune responses and promoting immunological tolerance. Related references: PMID:14556005
• Acts as a receptor for galectin-9 (LGALS9), which upon binding can induce apoptosis of antigen-specific T-cells, though this ligand-receptor interaction has been challenged. Related references: PMID:16286920, PMID:24337741, PMID:23555261
• Also binds phosphatidylserine (PtSer) on apoptotic cells, mediating their phagocytosis by dendritic cells and modulating innate immunity. (By similarity)
• Expressed on regulatory T (Treg) cells where it can inhibit Th17 cell responses; on natural killer (NK) cells, it has dual roles, either enhancing IFN-gamma production or suppressing cytotoxicity depending on context. Related references: PMID:24838857, PMID:22323453, PMID:22383801
• Subcellularly located at the cell membrane and cell junctions, where it interacts with signaling molecules like LCK and ZAP70 to modulate TCR signaling. Related references: PMID:24337741, PMID:26492563
• Tissue expression includes Th1 lymphocytes, Treg cells, dendritic cells, NK cells, and epithelial tissues; expression is upregulated in chronic viral infections such as HCV and HIV-1.
• Post-translational modifications include glycosylation, disulfide bond formation, palmitoylation, and phosphorylation, which regulate its localization and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–200) of human TIM-3/HAVCR2, which targets the native receptor on the cell surface—ideal for flow cytometry applications.
• For FC, titrate the antibody to determine the optimal staining concentration and include appropriate isotype controls to ensure specificity.
• TIM-3 expression is typically low on resting T-cells; consider activating cells (e.g., with anti-CD3/CD28) or using samples from chronic infection/tumor models to upregulate expression for detection.
• Validate performance in your cell system of interest; conditions such as sample preparation, fixation, and permeabilization may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers immunology and immuno-oncology research and IVD development with a one-stop supply chain for high-quality raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. This TIM-3/HAVCR2 antibody exemplifies our commitment to supporting immune checkpoint studies, offering reliable sourcing pathways for targets from early discovery to clinical assay development. Partner with CamelBio to streamline your procurement and accelerate your diagnostic innovation.