APC/Cyanine7 Rabbit anti-Human IFN-gamma Monoclonal Antibody for Flow Cytometry (Intracellular) - P01579

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• Interferon gamma (IFN-gamma), also known as immune interferon, is a type II interferon that plays critical roles in antimicrobial, antiviral, and antitumor immune responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:16914093, PMID:8666937
• Signals primarily via the JAK-STAT pathway through its receptor IFNGR1, leading to STAT1 activation and transcription of IFN-gamma-regulated genes. Related references: PMID:8349687, PMID:16914093
• Enhances MHC class I antigen presentation by inducing replacement of catalytic proteasome subunits with immunoproteasome subunits and increasing peptide generation efficiency. Related references: PMID:8666937, PMID:8163024, PMID:11112687
• Upregulates MHC class II complexes via promoting expression of cathepsins B, H, and L. Related references: PMID:7729559
• Participates in hematopoietic stem cell regulation, affecting development, quiescence, and differentiation.
• Primarily secreted from activated T lymphocytes, serving as a key cytokine in adaptive immunity.
• Annotated as a secreted glycoprotein with signal peptide, cleavage on pair of basic residues, and pyrrolidone carboxylic acid formation (UniProt keyword: Pyrrolidone carboxylic acid).

Experimental Guidance and Technical Tips

• This antibody is designed for intracellular flow cytometry (FC intra); ensure appropriate cell fixation and permeabilization protocols to detect cytoplasmic IFN-gamma.
• The immunogen spans amino acids 24–166 of the mature protein, covering the biologically active region; consider validating detection of both monomeric and dimeric forms in activated leukocyte samples.
• Conjugated to APC/Cyanine7, a far-red fluorophore; verify detector configuration and compensation settings for optimal signal separation.
• For induction of IFN-gamma expression in T-cells or NK cells, standard stimulation with PMA/ionomycin or specific antigens is recommended prior to intracellular staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories one-stop access to IVD raw materials, including this APC/Cyanine7-conjugated anti-human IFN-gamma monoclonal antibody. Our portfolio spans validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials, supporting immune response and cytokine research workflows from concept to clinic.