APC/Cyanine7 Rabbit anti-Human EGFR Monoclonal Antibody for FC - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase (RTK) of the ErbB family, also known as HER1 or c-ErbB-1, encoded by the EGFR gene.
• It binds EGF family ligands (e.g., EGF, TGF-alpha, AREG, epigen, betacellulin, epiregulin, HB-EGF) which trigger receptor dimerization and autophosphorylation. Related references: PMID:12297049 PMID:15611079 PMID:17909029
• Activated EGFR signals through RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, STATs, and NF-kappa-B cascades. Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:11116146
• It directly phosphorylates RGS16 and MUC1, linking to GPCR signaling and cell adhesion. Related references: PMID:11602604 PMID:11483589
• EGFR positively regulates cell migration via interaction with CCDC88A/GIV. Related references: PMID:20462955
• An alternative isoform (isoform 2) may antagonize EGF signaling.
• It localizes to the cell membrane, ER, Golgi, nucleus, endosome, and is secreted. Ubiquitously expressed; isoform 2 in ovarian cancers.
• The protein undergoes extensive post-translational modifications (glycosylation, phosphorylation, etc.) and acts as an HCV entry receptor.

Experimental Guidance and Technical Tips

• The immunogen (aa 335-525) lies within the extracellular ligand-binding domain, enabling detection of cell surface EGFR by flow cytometry without permeabilization.
• For flow cytometry, perform antibody titration to identify the optimal staining concentration for your specific cell type.
• Include appropriate isotype control and live/dead discrimination to ensure specific signal.
• As APC/Cyanine7 is a tandem dye, use appropriate compensation controls in multicolor panels; avoid prolonged light exposure and fixatives that may affect fluorophore stability.
• Validate staining specificity using EGFR-negative cell lines or ligand competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our EGFR antibody products support oncology and signaling research applications. From concept to clinic, we help diagnostic manufacturers and labs streamline their supply chain with reliable, high-quality reagents.