APC/Cyanine7 Rabbit anti-Human CD71 Monoclonal Antibody for FC - P02786

Core Product Specifications and Parameters

Biological Background: Transferrin Receptor Protein 1 (CD71) Function and Localization

• Transferrin receptor protein 1 (TFRC/CD71/T9/p90/TFR) is a type II transmembrane glycoprotein that mediates cellular iron uptake via receptor-mediated endocytosis of transferrin. After internalization, endosomal acidification releases iron, and the apotransferrin-receptor complex recycles to the cell surface. Related references: PMID:26214738, PMID:41772062
• TFRC is critical for the development of erythrocytes and the nervous system (By similarity).
• Functions as a lipid sensor that regulates mitochondrial fusion through the JNK pathway; low dietary stearate levels promote MFN2 degradation and inhibit fusion. Related references: PMID:26214738
• Positively regulates T and B cell proliferation via iron uptake. Related references: PMID:26642240
• Subcellularly localizes to the cell membrane, melanosomes, and is also secreted.
• Acts as a host cell receptor for multiple pathogens, including new-world arenaviruses (Guanarito, Junin, Machupo), rabies virus, SARS-CoV, MERS-CoV, SARS-CoV-2, and Plasmodium vivax.
• Post-translational modifications include disulfide bonds, glycosylation, palmitoylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC) with APC/Cyanine7 conjugation. Ensure appropriate laser (e.g., 633 nm) and emission filter (e.g., 780/60 nm) settings.
• The immunogen corresponds to the extracellular domain (aa 100–250) of human CD71, likely ensuring recognition of the native receptor on viable cells.
• For optimal FC staining, titrate the antibody and use appropriate positive (e.g., activated T cells) and negative controls.
• Cross-reactivity is listed as human; reactivity with other species should be validated independently.
• Store at 4°C protected from light to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined source for IVD raw materials, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. This anti-CD71 antibody supports research into iron metabolism, immunology, and infectious disease. For custom needs or rare target sourcing, CamelBio offers comprehensive procurement and technical support.