APC/Cyanine7 Rabbit anti-Human CD61 mAb for Flow Cytometry - P05106

Core Product Specifications and Parameters

Biological Background: CD61 (Integrin beta-3) Function and Localization

• CD61 (integrin beta-3) is a receptor subunit that pairs with ITGAV or ITGA2B to form functional integrins recognizing RGD motifs, mediating cell adhesion and signaling.
• In platelets, integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) mediates platelet aggregation through binding to soluble fibrinogen. Related references: PMID:9111081
• ITGAV:ITGB3 serves as a coreceptor for fractalkine (CX3CL1), essential for CX3CR1-dependent signaling. Related references: PMID:23125415, PMID:24789099
• ITGAV:ITGB3 binds growth factors such as FGF1, FGF2, IGF1, and IGF2, crucial for their signaling. Related references: PMID:18441324, PMID:28302677, PMID:19578119, PMID:28873464
• ITGAV:ITGB3 also interacts with CD40LG, IBSP, and fibrillin-1, supporting diverse cellular functions. Related references: PMID:31331973, PMID:10640428, PMID:12807887
• Subcellular localization includes the cell membrane, lamellipodium, focal adhesions, and postsynaptic membrane, reflecting roles in cell migration and synaptic function.
• Isoform beta-3A is widely expressed and specific in osteoblasts; isoform beta-3C is restricted to prostate and testis.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bonds, with an EGF-like domain critical for structure.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular domain (aa 21-718), suggesting recognition of native epitopes suitable for flow cytometry applications.
• As an APC/Cyanine7-conjugated antibody, it enables direct detection without secondary reagents; titrate to optimize signal-to-noise in your specific cell system.
• Validate in relevant human cell types (e.g., platelets, leukocytes, cancer cell lines) using appropriate isotype controls and Fc-blocking strategies.
• Consider that CD61 is highly expressed on platelets; careful gating or platelet depletion may be needed in whole blood assays to avoid interference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic and research applications. This anti-CD61 antibody supports studies in platelet biology, integrin signaling, and cardiovascular disease. Beyond individual reagents, we offer rare target sourcing and technical consulting to streamline your development from concept to clinic.