APC/Cyanine7 Rabbit anti-Human CD56/NCAM1 Monoclonal Antibody for Flow Cytometry - P13591

Core Product Specifications and Parameters

Biological Background: NCAM1 Function and Localization

• Neural cell adhesion molecule 1 (NCAM1) is a cell adhesion protein of the immunoglobulin superfamily, characterized by immunoglobulin-like domains and fibronectin type III repeats.
• Alternative splicing generates multiple isoforms, including GPI-anchored and transmembrane variants, and some secreted forms.
• NCAM1 mediates homophilic and heterophilic cell-cell adhesion, essential for neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.
• It additionally serves as a host cell receptor for rabies virus and Zika virus, facilitating viral entry.
• The protein is located primarily at the cell membrane, but secreted isoforms exist.
• Post-translational modifications include N-glycosylation, phosphorylation, disulfide bond formation, and GPI-anchor addition, which influence its structure and function.
• The theoretical molecular mass of the unprocessed precursor is ~94.6 kDa, but glycosylation increases the apparent weight to ~95 kDa and above.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant protein encompassing amino acids 20-413, representing the extracellular domain of human NCAM1. This design favors recognition of surface-expressed CD56 in flow cytometry.
• For flow cytometry, use the APC/Cyanine7 conjugate directly; consider titrating the antibody to determine the optimal working concentration for your cell type.
• Validate the antibody in relevant sample systems: CD56 is expressed in human natural killer cells and subsets of neurons, thus appropriate positive controls include human NK cell lines or peripheral blood mononuclear cells.
• Ensure your flow cytometer is equipped with an appropriate laser and filter set for APC-Cy7 detection (excitation ~633-650 nm, emission ~780 nm).
• Because the antibody is specific for human CD56, confirm cross-reactivity with your target species if working with non-human samples; cross-reactivity is reported for human only.
• For optimal performance, follow standard surface staining protocols and include appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This APC/Cyanine7 anti-human CD56 monoclonal antibody is part of our extensive portfolio for immunophenotyping and immune monitoring applications. We offer bulk quantities, customized formulation and conjugation services, and technical consultation to accelerate your assay development.